The selective retardation of NADP+-dependent dehydrogenases by immobilized procion red HE-3B
نویسندگان
چکیده
منابع مشابه
The selective retardation of NADP+-dependent dehydrogenases by immobilized procion red HE-3B.
The capacities of Procion Red HE-3B and Cibacron Blue F3G-A immobilized to Sepharose CL-4B and Matrex 201R for NAD+-, NADP+- and NAD(P)+-dependent dehydrogenases were measured. Procion Red HE-3B columns retarded NADP+-dependent dehydrogenases more effectively than NAD+-dependent dehydrogenases, whilst immobilized Cibacron Blue F3G-A retarded NAD+-dependent dehydrogenases more effectively than N...
متن کاملThe purification of nicotinamide nucleotide-dependent dehydrogenases on immobilized cofactors.
1. The general applicability of affinity chromatography to the purification of nicotinamide nucleotide-dependent dehydrogenases on immobilized cofactors is illustrated with several examples taken from crude systems. 2. Methods for overcoming the inevitable loss of selectivity experienced with these polymers are suggested. Effective use of the appropriate nucleotide, the second substrate and oth...
متن کاملRole of NADP-dependent and quinoprotein glucose dehydrogenases in gluconic acid production by G luconobacter oxydans
Gluconobacter oxydans, an organism used for the industrial production of gluconic acid, contains two glucose dehydrogenases (GDHs) catalysing the direct oxidation of glucose to gluconic acid. In addition to a quinoprotein (PQQ-dependent), membrane-bound GDH (EC 1.1.99.17), an NADP-dependent, cytoplasmic GDH is present. From three types of experiments, evidence is presented that the quinoprotein...
متن کاملPurification of hydrogenases by affinity chromatography on Procion Red-agarose.
The agarose-coupled triazine dye Procion Red HE-3B has been demonstrated to be applicable as an affinity gel for the purification of five diverse hydrogenases, namely the soluble, NAD-specific and the membrane-bound hydrogenase of Alcaligenes eutrophus, the membrane-bound hydrogenase of the N2-fixing Alcaligenes latus, the reversible H2-evolving and the unidirectional H2-oxidizing hydrogenase o...
متن کاملcontrol of the optical properties of nanoparticles by laser fields
در این پایان نامه، درهمتنیدگی بین یک سیستم نقطه کوانتومی دوگانه(مولکول نقطه کوانتومی) و میدان مورد مطالعه قرار گرفته است. از آنتروپی ون نیومن به عنوان ابزاری برای بررسی درهمتنیدگی بین اتم و میدان استفاده شده و تاثیر پارامترهای مختلف، نظیر تونل زنی(که توسط تغییر ولتاژ ایجاد می شود)، شدت میدان و نسبت دو گسیل خودبخودی بر رفتار درجه درهمتنیدگی سیستم بررسی شده اشت.با تغییر هر یک از این پارامترها، در...
15 صفحه اولذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1978
ISSN: 0264-6021
DOI: 10.1042/bj1730591